GeneTex
United States (US)

CXCR3 antibody [N1], N-term

GTX108145_39735_20150508_WB_19011507_298.jpg
GTX108145_41976_20150821_IHC-P_19011507_237.jpg
GTX108145_39735_20150806_IHC-P_19011507_899.jpg

Cat No. GTX108145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

K562 , NCI-H929

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CXCR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD182 antibody, CD183 antibody, CKR-L2 antibody, CMKAR3 antibody, GPR9 antibody, IP10-R antibody, Mig-R antibody, MigR antibody, CXCR3 antibody, CXC-R3 antibody, chemokine (C-X-C) receptor 3 antibody, C-X-C chemokine receptor type 3 antibody, IP10 receptor antibody, interferon-inducible protein 10 receptor antibody, G protein-coupled receptor 9 antibody, IP-10 receptor antibody, Mig receptor antibody, CXCR-3 antibody, chemokine (C-X-C motif) receptor 3 antibody

Cellular Localization

Cell membrane

Background

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108145_39735_20150508_WB_19011507_298.jpg

GTX108145 WB Image

CXCR3 antibody detects CXCR3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR3 antibody (GTX108145) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108145_41976_20150821_IHC-P_19011507_237.jpg

GTX108145 IHC-P Image

CXCR3 antibody [N1], N-term detects CXCR3 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CXCR3 antibody [N1], N-term (GTX108145) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108145_39735_20150806_IHC-P_19011507_899.jpg

GTX108145 IHC-P Image

CXCR3 antibody [N1], N-term detects CXCR3 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CXCR3 antibody [N1], N-term (GTX108145) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf