CXCR4 antibody [HL2612]

CXCR4 antibody [HL2612] detects CXCR4 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:500 and competitor's antibody (Highly Cited Competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

CXCR4 antibody [HL2612] detects CXCR4 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CXCR4 antibody [HL2612] detects CXCR4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR4 antibody [HL2612] detects CXCR4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR4 antibody [HL2612] detects CXCR4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR4 antibody [HL2612] (GTX639064) detects CXCR4 protein by flow cytometry analysis.
Sample: Jurkat and NCI-H460 cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.
The classification of protein expression as positive or negative was primarily based on data from the Human Protein Atlas (HPA).

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CXCR4 antibody [HL2612] detects CXCR4 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.