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CXCR4 antibody

Anti-CXCR4 antibody used in Western Blot (WB). GTX129719

Cat No. GTX129719

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

human CXCR4 -transfected 293T (unboiled)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody used in Western Blot (WB). GTX129719

GTX129719 WB Image

CXCR4 antibody detects CXCR4 protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody (GTX129719) at a dilution of 1:1000.

REFERENCE

There are currently no references for CXCR4 antibody (GTX129719). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CXCR4 antibody (GTX129719). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169