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CXCR4 antibody

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13854
Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX13854
Anti-CXCR4 antibody used in Western Blot (WB). GTX13854

Cat. No. GTX13854

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Sheep
Package
100 μg ($419)
See all CXCR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IHC-P 1:20 - 1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane,Cell junction,Lysosome

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13854

GTX13854 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX13854 CXCR4 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:25
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX13854

GTX13854 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX13854 CXCR4 antibody.
Dilution : 1:300

Anti-CXCR4 antibody used in Western Blot (WB). GTX13854

GTX13854 WB Image

WB analysis of HeLa cell lysate using GTX13854 CXCR4 antibody.
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419