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CXCR4 antibody

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22074
Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074
Anti-CXCR4 antibody used in Western Blot (WB). GTX22074
Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074
Anti-CXCR4 antibody used in Flow cytometry (FACS). GTX22074

Cat No. GTX22074

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 10 μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 0.1 μg/ml
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

Specificity/Sensitivity

CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence corresponding to amino acids 1-14 of human CD184.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane,Cell junction,Lysosome

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) using GTX22074 CXCR4 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of various sample lysates using GTX22074 CXCR4 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of CXCR4 partial recombinant protein using GTX22074 CXCR4 antibody.
Dilution : 0.1 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of various sample lysates using GTX22074 CXCR4 antibody.
Loading : 20μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22074

GTX22074 IHC-P Image

IHC-P analysis of human spleen tissue using GTX22074 CXCR4 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5 μg/ml

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074

GTX22074 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 2 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22074

GTX22074 WB Image

WB analysis of HeLa cell lysate using GTX22074 CXCR4 antibody.
Working concentration : 0.5 μg/ml

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22074

GTX22074 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 20 μg/ml

Anti-CXCR4 antibody used in Flow cytometry (FACS). GTX22074

GTX22074 FACS Image

FACS analysis of HeLa cells using GTX22074 CXCR4 antibody.
Working concentration : 0.1 μg/ml
Purple: cells without staining
Green: Isotype control
Red : CXCR4 antibody

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289