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CXCR4 antibody

KO/KD
Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22090
Anti-CXCR4 antibody used in Western Blot (WB). GTX22090
Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22090
Anti-CXCR4 antibody used in Western Blot (WB). GTX22090
Anti-CXCR4 antibody used in Western Blot (WB). GTX22090
Anti-CXCR4 antibody used in Western Blot (WB). GTX22090

Cat. No. GTX22090

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CXCR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CXCR4 antibody was raised against a 15 amino acid peptide near the center of human CXCR4.The immunogen is located within amino acids 170 - 220 of CXCR4(NP_003458).

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11163783

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane,Cell junction,Lysosome

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22090

GTX22090 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22090 CXCR4 antibody.
Working concentration : 4 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22090

GTX22090 WB Image

WB analysis of HeLa cell lysate using GTX22090 CXCR4 antibody.
Working concentration : 1 μg/ml

Anti-CXCR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX22090

GTX22090 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX22090 CXCR4 antibody.
Working concentration : 5 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22090

GTX22090 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) using GTX22090 CXCR4 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22090

GTX22090 WB Image

WB analysis of various sample lysates using GTX22090 CXCR4 antibody.
Loading : 20μg of lysates per lane
Dilution : 2 μg/ml

Anti-CXCR4 antibody used in Western Blot (WB). GTX22090

GTX22090 WB Image

WB analysis of various sample lysates using GTX22090 CXCR4 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

REFERENCE

Application Reference

Chiang HY et al. PLoS One 2017; 12 (10) : e0185811 R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399