CXCR5 antibody [C1C2], Internal

Name: CXCR5 antibody [C1C2], Internal
Brand: GeneTex
SKU: GTX100351
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CXCR5 antibody [C1C2], Internal used in Western Blot (WB). GTX100351

Anti-CXCR5 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100351

Cat. No. GTX100351

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CXCR5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240668

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 5 , BLR1 , CD185 , MDR15

Cellular Localization

Cell membrane

Background

This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq]

Database

Gene ID: 643 CXCR5
UniProt: P32302 CXCR5

Research Area

DATA IMAGES

GTX100351 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX100351 diluted at 1:1000

GTX100351 ICC/IF Image

CXCR5 antibody [C1C2], Internal detects CXCR5 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR5 stained by CXCR5 antibody [C1C2], Internal (GTX100351) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Kogami A et al. J Histochem Cytochem 2021; 69 (10) : 645-657 The Conspicuousness of High Endothelial Venules in Angioimmunoblastic T-cell Lymphoma Is Due to Increased Cross-sectional Area, Not Increased Distribution Density.

Calvet-Mirabent M et al. Eur J Immunol 2021; (Epub) Poly I:C and STING agonist-primed DC increase lymphoid tissue polyfunctional HIV-1-specific CD8⁺ T cells and limit CD4⁺ T-cell loss in BLT mice.

Reactivity : Mouse

Chao CC et al. J Cell Mol Med 2021; 25 (19) : 9128-9140 CXC chemokine ligand-13 promotes metastasis via CXCR5-dependent signaling pathway in non-small cell lung cancer.

Application : WB

Reactivity : Human

Lin JR et al. Elife 2018; 7 Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Yang H et al. Mol Med Rep. 2017; (Epub) Frequency and distribution of CD4+CXCR5+ follicular B helper T cells within involved tissues in IgG4-related ophthalmic disease.

Reactivity : Human

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