GeneTex
United States (US)

CXCR5 antibody [C1C2], Internal

GTX100351_39680_IHC_19011506_705.jpg
GTX100351_WB_19011506_470.jpg

Cat No. GTX100351

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BLR1 antibody, CD185 antibody, MDR15 antibody, MGC117347 antibody, CXCR5 antibody, monocyte-derived receptor 15 antibody, Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody, C-X-C chemokine receptor type 5 antibody, CXC-R5 antibody, Burkitt lymphoma receptor 1, GTP-binding protein antibody, MDR-15 antibody, CXCR-5 antibody, chemokine (C-X-C motif) receptor 5 antibody

Cellular Localization

Cell membrane

Background

This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq]

Database

DATA IMAGES
GTX100351_39680_IHC_19011506_705.jpg

GTX100351 IHC-P Image

CXCR5 antibody [C1C2], Internal detects CXCR5 protein at membrane on PC13 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC13 xenograft.
CXCR5 antibody [C1C2], Internal (GTX100351) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100351_WB_19011506_470.jpg

GTX100351 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX100351 diluted at 1:1000

REFERENCE
REVIEW

CXCR5 antibody [C1C2], Internal Cat No. GTX100351

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169