CXCR5 antibody [HL3708]

CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse colon.
Green: CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250.
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) unboiled Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.