CXCR5 antibody [HL3778]

CXCR5 antibody [HL3778] detects CXCR5 protein by immunofluorescent analysis.
Sample: Daudi cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR5 stained by CXCR5 antibody [HL3778] (GTX641977) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

CXCR5 antibody [HL3778] detects CXCR5 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR5 stained by CXCR5 antibody [HL3778] (GTX641977) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CXCR5 antibody [HL3778] antibody (GTX641977) detects CXCR5 protein by flow cytometry analysis.
Sample: Non-fixed and non-permeabilized Raji or Jurkat cells were stained with 5μg/ml GTX641977 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4ºC for 1 hour. Alexa Fluor 488-labeled anti-Rabbit IgG at 1/500 dilution was used as the secondary antibody. Prior to staining process, Raji or Jurkat cells were treated with the FcR Blocking Reagent 10 mins at 4ºC.