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CXCR5 antibody

Anti-CXCR5 antibody used in Flow cytometry (FACS). GTX04093
Anti-CXCR5 antibody used in Western Blot (WB). GTX04093

Cat. No. GTX04093

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, FACS, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
FACS 1-3 μg/1x106 cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human CXCR5 recombinant protein (Position: A181-S358).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 5 , BLR1 , CD185 , MDR15

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CXCR5 antibody used in Flow cytometry (FACS). GTX04093

GTX04093 FACS Image

FACS analysis of HEL cells using GTX04093 CXCR5 antibody.
Dilution : 1 μg/1x106
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-CXCR5 antibody used in Western Blot (WB). GTX04093

GTX04093 WB Image

WB analysis of various cell lysates using GTX04093 CXCR5 antibody.
Lane 1 : Raji cell lysates
Lane 2 : MOLT-4 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : rat brain tissue lysates
Lane 5 : mouse brain tissue lysates
Lane 6 : mouse lung tissue lysates
Dilution : 0.5 μg/mL
Loading : 30 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 429