CXCR6 antibody [HL2849]

CXCR6 antibody [HL2849] (GTX640120) detects CXCR6 protein by flow cytometry analysis.
Sample: Jurkat were blocked with an FcR blocking reagent for 10 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CXCR6 antibody [HL2849] (GTX640120) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

CXCR6 antibody [HL2849] detects CXCR6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR6 stained by CXCR6 antibody [HL2849] (GTX640120) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CXCR6 antibody [HL2849] detects CXCR6 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR6 stained by CXCR6 antibody [HL2849] (GTX640120) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR6 antibody [HL2849] (GTX640120) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.