GeneTex
United States (US)

CXCR7 antibody [C1C2], Internal

GTX100027_IHC_19011506_627.jpg
GTX100027_IHC1_19011506_368.jpg
GTX100027_IHC2_19011506_934.jpg
GTX100027_39210_WB_19011506_173.jpg
GTX100027_IFA_19011506_646.jpg

Cat No. GTX100027

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

PC-3 , U87-MG , *human CXCR7 transfected Mesenchymal stem cells

Predict Reactivity

Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CMKOR1 antibody, GPR159 antibody, RDC1 antibody, CXCR7 antibody, CXCR-7 antibody, G protein-coupled receptor antibody, chemokine orphan receptor 1 antibody, CXC-R7 antibody, G-protein coupled receptor RDC1 homolog antibody, G-protein coupled receptor 159 antibody, C-X-C chemokine receptor type 7 antibody, RDC-1 antibody, chemokine (C-X-C motif) receptor 7 antibody

Cellular Localization

Cell membrane

Background

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100027_IHC_19011506_627.jpg

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027_IHC1_19011506_368.jpg

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027_IHC2_19011506_934.jpg

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027_39210_WB_19011506_173.jpg

GTX100027 WB Image

Sample (30 µg of whole cell lysate)
A: PC-3
B: U87-MG
10% SDS PAGE
GTX100027 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100027_IFA_19011506_646.jpg

GTX100027 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human pancreatic cancer cell line, using CXCR7(GTX100027) antibody at 1:50 dilution. Blue: DAPI.

REFERENCE
REVIEW

CXCR7 antibody [C1C2], Internal Cat No. GTX100027

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CXCR7 transfected Mesenchymal stem cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Mesenchymal stem cells.Lane 2: Mesenchymal stem cells transfected with human CXCR7 protein.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169