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CXCR7 antibody [C1C2], Internal

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027
Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

Cat. No. GTX100027

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

CXCR7 antibody recognizes C-X-C chemokine receptor type 7 (CXCR7), now known as atypical chemokine receptor 3 (ACKR3). This chemokine receptor (with a predicted molecular weight of 41 kDa) binds the chemokines CXCL11 and CXCL12 and other non-chemokine ligands such as adrenomedullin and MIF. ACKR3 plays important roles in cardiovascular and neuronal development and acts as a scavenger receptor to regulate both chemokine- and opioid peptide-dependent signaling.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

atypical chemokine receptor 3 , CMKOR1 , CXC-R7 , CXCR-7 , CXCR7 , GPR159 , RDC-1 , RDC1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCR7 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100027

GTX100027 ICC/IF Image

CXCR7 antibody [C1C2], Internal detects CXCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCR7 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100027

GTX100027 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human pancreatic cancer cell line, using CXCR7(GTX100027) antibody at 1:50 dilution. Blue: DAPI.

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

Boiled and unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32366833

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32366833

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32366833

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

The data was published in the journal Stem Cells Int in 2014. PMID: 24757448

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

GTX100027 IHC-P Image

The data was published in the journal Cell Death Dis in 2019.PMID: 30804329

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

GTX100027 WB Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32366833

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

GTX100027 IHC-P Image

The data was published in the journal Mol Cancer in 2014. PMID: 25168820

REFERENCE

REVIEW

CXCR7 antibody [C1C2], Internal Cat. No. GTX100027

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CXCR7 transfected Mesenchymal stem cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Mesenchymal stem cells.Lane 2: Mesenchymal stem cells transfected with human CXCR7 protein.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169