CXCR7 antibody [C1C2], Internal

Name: CXCR7 antibody [C1C2], Internal
Brand: GeneTex
SKU: GTX100027
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CXCR7 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100027

Anti-CXCR7 antibody [C1C2], Internal used in Western Blot (WB). GTX100027

Anti-CXCR7 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100027

Cat. No. GTX100027

Reference 25

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all CXCR7 products

View product citations for antibody GTX100027 on CiteAb

PRODUCT

Summary

CXCR7 antibody recognizes C-X-C chemokine receptor type 7 (CXCR7), now known as atypical chemokine receptor 3 (ACKR3). This chemokine receptor (with a predicted molecular weight of 41 kDa) binds the chemokines CXCL11 and CXCL12 and other non-chemokine ligands such as adrenomedullin and MIF. ACKR3 plays important roles in cardiovascular and neuronal development and acts as a scavenger receptor to regulate both chemokine- and opioid peptide-dependent signaling.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

atypical chemokine receptor 3 , CMKOR1 , CXC-R7 , CXCR-7 , CXCR7 , GPR159 , RDC-1 , RDC1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq]

Database

Gene ID: 57007 ACKR3
UniProt: P25106 ACKR3

Research Area

DATA IMAGES

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027 WB Image

Boiled and unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

GTX100027 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100027 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100027 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100027 ICC/IF Image

CXCR7 antibody [C1C2], Internal detects CXCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100027 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CXCR7(GTX100027) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100027 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human pancreatic cancer cell line, using CXCR7(GTX100027) antibody at 1:50 dilution. Blue: DAPI.

GTX100027 WB Image

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal (GTX100027) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.