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CXCR7 antibody [HL2189]

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

Cat. No. GTX638193

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2189

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody was raised against human CXCR7 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR7. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

atypical chemokine receptor 3 , CMKOR1 , CXC-R7 , CXCR-7 , CXCR7 , GPR159 , RDC-1 , RDC1

Cellular Localization

Cell membrane; Multi-pass membrane protein,Cytoplasm, perinuclear region,Early endosome

Background

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:200 and competitor's antibody (MAB42273) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCR7 antibody [HL2189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638193

GTX638193 ICC/IF Image

CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations