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CYB5R3 antibody [1E3]

Overexpression
Anti-CYB5R3 antibody [1E3] used in Flow cytometry (FACS). GTX84645
Anti-CYB5R3 antibody [1E3] used in Western Blot (WB). GTX84645
Anti-CYB5R3 antibody [1E3] used in IHC (Paraffin sections) (IHC-P). GTX84645
Anti-CYB5R3 antibody [1E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84645
Anti-CYB5R3 antibody [1E3] used in Western Blot (WB). GTX84645

Cat. No. GTX84645

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all CYB5R3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b5 reductase 3 , B5R , DIA1

Background

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Database

Research Area

DATA IMAGES

Anti-CYB5R3 antibody [1E3] used in Flow cytometry (FACS). GTX84645

GTX84645 FACS Image

FACS analysis of HEK293T cells transfected with either CYB5R3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84645 CYB5R3 antibody [1E3].

Anti-CYB5R3 antibody [1E3] used in Western Blot (WB). GTX84645

GTX84645 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CYB5R3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84645 CYB5R3 antibody [1E3].
Loading : 5 ug per lane

Anti-CYB5R3 antibody [1E3] used in IHC (Paraffin sections) (IHC-P). GTX84645

GTX84645 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX84645 CYB5R3 antibody [1E3].

Anti-CYB5R3 antibody [1E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84645

GTX84645 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CYB5R3 plasmid using GTX84645 CYB5R3 antibody [1E3].

Anti-CYB5R3 antibody [1E3] used in Western Blot (WB). GTX84645

GTX84645 WB Image

WB analysis of various cell lines using GTX84645 CYB5R3 antibody [1E3].
Loading : 35 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519