CYB5R3 antibody [2A10]

Cat. No. GTX84646

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	2A10
Isotype	IgG2b
Application	WB, IHC-P, FACS, IP
Reactivity	Human, Dog, Monkey
Package	100 μl ($519)

See all CYB5R3 products

View product citations for antibody GTX84646 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
IHC-P	1:150
FACS	1:100
IP	Assay dependent

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b5 reductase 3 , B5R , DIA1

Background

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Database

Gene ID: 1727 CYB5R3
UniProt: P00387 CYB5R3

Research Area

DATA IMAGES

GTX84646 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 FACS Image

FACS analysis of Jurkat cells using GTX84646 CYB5R3 antibody [2A10].
Red : Primary antibody
Blue : Negative control antibody

GTX84646 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 WB Image

WB analysis of HEK293T cells transfected with CYB5R3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84646 CYB5R3 antibody [2A10].
Loading : 5 ug per lane

GTX84646 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 IHC-P Image

IHC-P analysis of human liver tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 WB Image

WB analysis of various cell lines using GTX84646 CYB5R3 antibody [2A10].
Loading : 35 ug per lane

GTX84646 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84646 FACS Image

FACS analysis of HEK293T cells transfected with either CYB5R3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84646 CYB5R3 antibody [2A10].

GTX84646 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84646 CYB5R3 antibody [2A10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Ishimura R et al. Nat Commun 2022; 13 (1) : 7857 The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Application : WB, IP

Reactivity : Human

Submit a Reference

REVIEW

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Submit a Review

Application Reference

Ishimura R et al. Nat Commun 2022; 13 (1):7857 The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Application : WB, IP

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

CYB5R3 antibody [2A10]

Cat. No. GTX84646

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX84646 IHC-P Image

GTX84646 FACS Image

GTX84646 IHC-P Image

GTX84646 IHC-P Image

GTX84646 WB Image

GTX84646 IHC-P Image

GTX84646 IHC-P Image

GTX84646 IHC-P Image

GTX84646 IHC-P Image

GTX84646 IHC-P Image

GTX84646 WB Image

GTX84646 IHC-P Image

GTX84646 FACS Image

GTX84646 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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