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CYFIP1 antibody [N1N2], N-term

Anti-CYFIP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122467
Anti-CYFIP1 antibody [N1N2], N-term used in Western Blot (WB). GTX122467
Anti-CYFIP1 antibody [N1N2], N-term used in Western Blot (WB). GTX122467
Anti-CYFIP1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX122467
Anti-CYFIP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122467

Cat. No. GTX122467

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CYFIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CYFIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164984

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytoplasmic FMR1 interacting protein 1 , P140SRA-1 , SHYC , SRA-1 , SRA1

Cellular Localization

Cytoplasm , perinuclear region , Cell projection , lamellipodium , ruffle , Cell junction , synapse , synaptosome

Background

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.

Database

Research Area

DATA IMAGES

Anti-CYFIP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122467

GTX122467 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYFIP1(GTX122467) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CYFIP1 antibody [N1N2], N-term used in Western Blot (WB). GTX122467

GTX122467 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX122467 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYFIP1 antibody [N1N2], N-term used in Western Blot (WB). GTX122467

GTX122467 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term (GTX122467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYFIP1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX122467

GTX122467 IP Image

Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 μg of CYFIP1 antibody [N1N2], N-term (GTX122467).
Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term (GTX122467).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CYFIP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122467

GTX122467 ICC/IF Image

CYFIP1 antibody [N1N2], N-term detects CYFIP1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYFIP1 stained by CYFIP1 antibody [N1N2], N-term (GTX122467) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Majumder P et al. Acta Neuropathol 2016; 132 (5) : 721-738 Co-regulation of mRNA translation by TDP-43 and Fragile X Syndrome protein FMRP.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169