APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYLD. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910034
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CYLD lysine 63 deubiquitinase , BRSS , CDMT , CYLD1 , CYLDI , EAC , MFT , MFT1 , SBS , TEM , USPL2
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton
Background
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662](/templates/default/js/lightbox/images/loading.gif "Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662")
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GTX636662 ICC/IF Image
CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:685.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636662 IHC-P Image
CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min
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GTX636662 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636662 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CYLD antibody [HL1260] (GTX636662). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CYLD antibody [HL1260] (GTX636662). Be the first to share your experience with this product.
![Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662](/upload/website/prouct_img/small/GTX636662/GTX636662_T-44529_20220916_ICC_IF_22102723_937.webp "Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662")
![Anti-CYLD antibody [HL1260] used in IHC (Paraffin sections) (IHC-P). GTX636662](/upload/website/prouct_img/small/GTX636662/GTX636662_44613_20220610_IHC-P_22062121_730.webp "Anti-CYLD antibody [HL1260] used in IHC (Paraffin sections) (IHC-P). GTX636662")
![Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662](/upload/website/prouct_img/small/GTX636662/GTX636662_T-44529_20220114_WB_competitor_watermark_w_23061202_889.webp "Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662")
![Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662](/upload/website/prouct_img/small/GTX636662/GTX636662_44613_20220311_WB_M_R_w_23061202_188.webp "Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662")
![Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662](/upload/website/prouct_img/normal/GTX636662/GTX636662_T-44529_20220916_ICC_IF_22102723_937.webp "Anti-CYLD antibody [HL1260] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636662")
![Anti-CYLD antibody [HL1260] used in IHC (Paraffin sections) (IHC-P). GTX636662](/upload/website/prouct_img/normal/GTX636662/GTX636662_44613_20220610_IHC-P_22062121_730.webp "Anti-CYLD antibody [HL1260] used in IHC (Paraffin sections) (IHC-P). GTX636662")
![Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662](/upload/website/prouct_img/normal/GTX636662/GTX636662_T-44529_20220114_WB_competitor_watermark_w_23061202_889.webp "Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662")
![Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662](/upload/website/prouct_img/normal/GTX636662/GTX636662_44613_20220311_WB_M_R_w_23061202_188.webp "Anti-CYLD antibody [HL1260] used in Western Blot (WB). GTX636662")