Immunofluorescence analysis of methanol-fixed HeLa, using Cylindromatosis 1(GTX113662) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [N1N2], N-term (GTX113662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.