*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Mouse, Rat, Bovine(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.89 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human CYLD. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CYLD lysine 63 deubiquitinase , BRSS , CDMT , CYLD1 , CYLDI , EAC , MFT , MFT1 , SBS , TEM , USPL2
Cytoplasm , perinuclear region
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
GTX113662 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [N1N2], N-term (GTX113662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX113662 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Cylindromatosis 1(GTX113662) antibody at 1:200 dilution.