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CYLD antibody

Anti-CYLD antibody used in Western Blot (WB). GTX100228
Anti-CYLD antibody used in Western Blot (WB). GTX100228
Anti-CYLD antibody used in IHC (Paraffin sections) (IHC-P). GTX100228
Anti-CYLD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100228
Anti-CYLD antibody used in Western Blot (WB). GTX100228

Cat No. GTX100228

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

HepG2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYLD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CYLD lysine 63 deubiquitinase,BRSS,CDMT,CYLD1,CYLDI,EAC,MFT,MFT1,SBS,TEM,USPL2

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CYLD antibody used in Western Blot (WB). GTX100228

GTX100228 WB Image

CYLD antibody detects CYLD protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
CYLD antibody (GTX100228) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYLD antibody used in Western Blot (WB). GTX100228

GTX100228 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX100228 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYLD antibody used in IHC (Paraffin sections) (IHC-P). GTX100228

GTX100228 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using cylindromatosis 1(GTX100228) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CYLD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100228

GTX100228 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using cylindromatosis 1(GTX100228) antibody at 1:200 dilution.

Anti-CYLD antibody used in Western Blot (WB). GTX100228

GTX100228 WB Image

CYLD antibody detects CYLD protein by western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
CYLD antibody (GTX100228) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169