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CYP11A1 antibody

Anti-CYP11A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX53941
Anti-CYP11A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53941
Anti-CYP11A1 antibody used in Western Blot (WB). GTX53941

Cat. No. GTX53941

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CYP11A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:20 - 1:50
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CYP11A1 (NP_000772.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 11 subfamily A member 1 , CYP11A , CYPXIA1 , P450SCC

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP11A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX53941

GTX53941 IHC-P Image

IHC-P analysis of human colon tissue using GTX53941 CYP11A1 antibody.
Dilution : 1:100

Anti-CYP11A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53941

GTX53941 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX53941 CYP11A1 antibody.
Blue : DAPI for nuclear staining

Anti-CYP11A1 antibody used in Western Blot (WB). GTX53941

GTX53941 WB Image

WB analysis of various sample lysates using GTX53941 CYP11A1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399