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CYP11A1 antibody

Anti-CYP11A1 antibody used in Western Blot (WB). GTX56293
Anti-CYP11A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56293

Cat. No. GTX56293

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CYP11A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of CYP11A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2801476

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome P450 family 11 subfamily A member 1 , CYP11A , CYPXIA1 , P450SCC

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP11A1 antibody used in Western Blot (WB). GTX56293

GTX56293 WB Image

WB analysis of Jurkat (A), mouse liver (B), rat liver (C) whole cell lysates using GTX56293 CYP11A1 antibody.

Anti-CYP11A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56293

GTX56293 ICC/IF Image

ICC/IF analysis of formalin-fixed Jurkat cells using GTX56293 CYP11A1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

Application Reference

Pooja et al. High Alt Med Biol 2021; Association Between 17β-Estradiol Receptors and Nitric Oxide Signaling Augments High-Altitude Adaptation of Ladakhi Highlanders.
Feng X et al. Mol Cell Endocrinol 2021; 519 : 111039 Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
Application : ICC/IF
Reactivity : Human
Zhang C et al. Aging (Albany NY) 2020; 12 (2) : 1272-1284 FOXO4-DRI alleviates age-related testosterone secretion insufficiency by targeting senescent Leydig cells in aged mice.
Pooja et al. Nitric Oxide 2020; 102 : 12-20 Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS-NO-cGMP pathway facilitates high altitude acclimatization.
Application : WB
Reactivity : Human
Kurniawan AL et al. Food Funct 2019; 10 (7) : 4113-4123 Alteration in iron efflux affects male sex hormone testosterone biosynthesis in a diet-induced obese rat model.
Application : WB
Reactivity : Rat
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399