APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 3-5μg/ml |
|
Note :
IHC-P
Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89365-PEP).
Predict Reactivity
Cat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-LFKDGDQKLEK, from the internal region of the protein sequence according to NP_000093.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 17 subfamily A member 1 , CPT7 , CYP17 , P450C17 , S17AH
Cellular Localization
Membrane
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89365 WB Image
WB analysis of human ovary lysate using GTX89365 CYP17A1 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
|
|
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GTX89365 IHC-P Image
IHC-P analysis of human adrenal gland using GTX89365 CYP17A1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for CYP17A1 antibody, Internal (GTX89365). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CYP17A1 antibody, Internal (GTX89365). Be the first to share your experience with this product.
