GeneTex
United States (US)

CYP17A1 antibody [N1C2]

GTX102753_40527_20160128_WB_M_19011506_333.jpg
GTX102753_40527_WB_19011506_147.jpg
GTX102753_40527_20160121_WB_19011506_858.jpg

Cat No. GTX102753

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

NIH-3T3 , C2C12 , MCF-7 , HepG2

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP17A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome P450 Family 17 Subfamily A Member 1,Cpt7,Cyp17,P450C17,S17Ah,Cyp17A1

Cellular Localization

Membrane , Membrane; Single-pass membrane protein , Membrane , Membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102753_40527_20160128_WB_M_19011506_333.jpg

GTX102753 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] (GTX102753) diluted at 1:1000.

GTX102753_40527_WB_19011506_147.jpg

GTX102753 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX102753 diluted at 1:10000

GTX102753_40527_20160121_WB_19011506_858.jpg

GTX102753 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] (GTX102753) diluted at 1:1000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169