APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , HepG2 , NIH-3T3 , C2C12
Predict Reactivity
Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CYP17A1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11165510
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 17 subfamily A member 1 , CPT7 , CYP17 , P450C17 , S17AH
Cellular Localization
Membrane , Membrane; Single-pass membrane protein , Membrane , Membrane
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102753 WB Image
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE GTX102753 diluted at 1:10000
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GTX102753 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] (GTX102753) diluted at 1:1000.
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GTX102753 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] (GTX102753) diluted at 1:1000.
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REFERENCE
There are currently no references for CYP17A1 antibody [N1C2] (GTX102753). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CYP17A1 antibody [N1C2] (GTX102753). Be the first to share your experience with this product.