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CYP17A1 antibody [N3C2], Internal

Anti-CYP17A1 antibody [N3C2], Internal used in Western Blot (WB). GTX113670

Cat. No. GTX113670

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CYP17A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , Molt-4

Predict Reactivity

Cat, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP17A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036721

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 17 subfamily A member 1 , CPT7 , CYP17 , P450C17 , S17AH

Cellular Localization

Membrane , Membrane; Single-pass membrane protein , Membrane , Membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CYP17A1 antibody [N3C2], Internal used in Western Blot (WB). GTX113670

GTX113670 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX113670 diluted at 1:1000

REFERENCE

Application Reference

Lin CJ et al. Biochem Pharmacol 2014; (Epub) Assessment of the potential of polyphenols as a CYP17 inhibitor free of adverse corticosteroid elevation.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169