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CYP1A2 antibody [7D12]

Anti-CYP1A2 antibody [7D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84643
Anti-CYP1A2 antibody [7D12] used in IHC (Paraffin sections) (IHC-P). GTX84643
Anti-CYP1A2 antibody [7D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84643
Anti-CYP1A2 antibody [7D12] used in Western Blot (WB). GTX84643
Anti-CYP1A2 antibody [7D12] used in IHC (Paraffin sections) (IHC-P). GTX84643
Anti-CYP1A2 antibody [7D12] used in Flow cytometry (FACS). GTX84643

Cat. No. GTX84643

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7D12

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all CYP1A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 1 subfamily A member 2 , CP12 , CYPIA2 , P3-450 , P450(PA)

Background

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.

Database

Research Area

DATA IMAGES

Anti-CYP1A2 antibody [7D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84643

GTX84643 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CYP1A2 plasmid using GTX84643 CYP1A2 antibody [7D12].

Anti-CYP1A2 antibody [7D12] used in IHC (Paraffin sections) (IHC-P). GTX84643

GTX84643 IHC-P Image

IHC-P analysis of human liver tissue using GTX84643 CYP1A2 antibody [7D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CYP1A2 antibody [7D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84643

GTX84643 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84643 CYP1A2 antibody [7D12].

Anti-CYP1A2 antibody [7D12] used in Western Blot (WB). GTX84643

GTX84643 WB Image

WB analysis of HEK293T cells transfected with CYP1A2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84643 CYP1A2 antibody [7D12].
Loading : 5 ug per lane

Anti-CYP1A2 antibody [7D12] used in IHC (Paraffin sections) (IHC-P). GTX84643

GTX84643 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84643 CYP1A2 antibody [7D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CYP1A2 antibody [7D12] used in Flow cytometry (FACS). GTX84643

GTX84643 FACS Image

FACS analysis of HEK293T cells transfected with either CYP1A2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84643 CYP1A2 antibody [7D12].

REFERENCE

Application Reference

Michielin F et al. Cell Rep 2020; 33 (9) : 108453 The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519