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CYP1B1 antibody

Anti-CYP1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104424
Anti-CYP1B1 antibody used in Western Blot (WB). GTX104424
Anti-CYP1B1 antibody used in Western Blot (WB). GTX104424

Cat No. GTX104424

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , mouse lung

Predict Reactivity

Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cytochrome P450 family 1 subfamily B member 1 , ASGD6 , CP1B , CYPIB1 , GLC3A , P4501B1

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CYP1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104424

GTX104424 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytochrome P450 1B1(GTX104424) antibody at 1:200 dilution.

Anti-CYP1B1 antibody used in Western Blot (WB). GTX104424

GTX104424 WB Image

CYP1B1 antibody detects CYP1B1 protein by western blot analysis.
A. 50 µg mouse lung lysate/extract
7.5% SDS-PAGE
CYP1B1 antibody (GTX104424) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP1B1 antibody used in Western Blot (WB). GTX104424

GTX104424 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP1B1 antibody (GTX104424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169