GeneTex
United States (US)

CYP24A1 antibody

GTX105884_39953_IHC_M_19011506_390.jpg
GTX105884_WB_19011506_395.jpg

Cat No. GTX105884

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP24A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CP24 antibody, CYP24 antibody, MGC126273 antibody, MGC126274 antibody, P450-CC24 antibody, CYP24A1 antibody, 24-OHase antibody, cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) antibody, cytochrome P450-CC24 antibody, vitamin D(3) 24-hydroxylase antibody, cytochrome P450 24A1 antibody, vitamin D 24-hydroxylase antibody, exo-mitochondrial protein antibody, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial antibody, 1,25-@dihydroxyvitamin D3 24-hydroxylase antibody, cytochrome P450, family 24, subfamily A, polypeptide 1 antibody

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX105884_39953_IHC_M_19011506_390.jpg

GTX105884 IHC-P Image

CYP24A1 antibody detects CYP24A1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CYP24A1 antibody (GTX105884) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105884_WB_19011506_395.jpg

GTX105884 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX105884 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf