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CYP27A1 antibody [HL1820]

Recombinant antibody Orthogonal
Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545
Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545
Anti-CYP27A1 antibody [HL1820] used in IHC (Paraffin sections) (IHC-P). GTX637545
Anti-CYP27A1 antibody [HL1820] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637545
Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545
Anti-CYP27A1 antibody [HL1820] used in IHC (Paraffin sections) (IHC-P). GTX637545

Cat. No. GTX637545

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1820

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CYP27A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CYP27A1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome P450 family 27 subfamily A member 1 , CP27 , CTX , CYP27

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545

GTX637545 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545

GTX637545 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP27A1 antibody [HL1820] used in IHC (Paraffin sections) (IHC-P). GTX637545

GTX637545 IHC-P Image

CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CYP27A1 antibody [HL1820] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637545

GTX637545 ICC/IF Image

CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CYP27A1 antibody [HL1820] used in Western Blot (WB). GTX637545

GTX637545 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CYP27A1 antibody [HL1820] used in IHC (Paraffin sections) (IHC-P). GTX637545

GTX637545 IHC-P Image

CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations