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CYP27A1 antibody

Orthogonal
Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718
Anti-CYP27A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103718
Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718
Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718
Anti-CYP27A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103718
Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718

Cat. No. GTX103718

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CYP27A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Mouse liver , rat liver , A549

Predict Reactivity

Rabbit, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168982

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 27 subfamily A member 1 , CP27 , CTX , CYP27

Cellular Localization

Mitochondrion membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718

GTX103718 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody (GTX103718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CYP27A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103718

GTX103718 IHC-P Image

CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CYP27A1 antibody (GTX103718) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718

GTX103718 WB Image

Sample (50 μg of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718

GTX103718 WB Image

CYP27A1 antibody detects CYP27A1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
7.5% SDS-PAGE
CYP27A1 antibody (GTX103718) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP27A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103718

GTX103718 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CYP27A1(GTX103718) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CYP27A1 antibody used in Western Blot (WB). GTX103718

GTX103718 WB Image

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kim MH et al. PLoS One 2023; 18 (6) : e0287146 Macrophage inhibitory cytokine-1 aggravates diet-induced gallstone formation via increased ABCG5/ABCG8 expression.
Lee EJ et al. Int J Mol Med 2017; (Epub) Proteasome inhibition protects against diet-induced gallstone formation through modulation of cholesterol and bile acid homeostasis.
Sharanek A et al. Toxicol Sci 2015; 147 (2) : 573-87 cellular accumulation and toxic effects of bile acids in * a-treated heparg hepatocytes.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169