Sample (50 ug of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX114065 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody (GTX114065) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded hela xenograft, using CYP27A1 antibody(GTX114065) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min