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CYP27B1 antibody

Anti-CYP27B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00645
Anti-CYP27B1 antibody used in Western Blot (WB). GTX00645

Cat No. GTX00645

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-195 of human CYP27B1 (NP_000776.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CP2B , CYP1 , CYP1alpha , CYP27B , CYP27B1 , P450c1 , PDDR , VDD1 , VDDR , VDDRI , VDR , cytochrome P450 family 27 subfamily B member 1

Cellular Localization

Mitochondrion membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP27B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00645

GTX00645 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00645 CYP27B1 antibody.
Red : Primary antibody
Blue: DAPI for nuclear staining
Dilution : 1:100

Anti-CYP27B1 antibody used in Western Blot (WB). GTX00645

GTX00645 WB Image

WB analysis of various samples using GTX00645 CYP27B1 antibody.
Dilution : 1:3000 
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319