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CYP2E1 antibody [5F11]

Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634
Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634
Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634
Anti-CYP2E1 antibody [5F11] used in Western Blot (WB). GTX84634
Anti-CYP2E1 antibody [5F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84634

Cat. No. GTX84634

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all CYP2E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 2 subfamily E member 1 , CPE1 , CYP2E , P450-J , P450C2E

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion inner membrane

Background

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.

Database

Research Area

DATA IMAGES

Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634

GTX84634 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84634 CYP2E1 antibody [5F11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634

GTX84634 IHC-P Image

IHC-P analysis of ovary tissue using GTX84634 CYP2E1 antibody [5F11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CYP2E1 antibody [5F11] used in IHC (Paraffin sections) (IHC-P). GTX84634

GTX84634 IHC-P Image

IHC-P analysis of liver tissue using GTX84634 CYP2E1 antibody [5F11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CYP2E1 antibody [5F11] used in Western Blot (WB). GTX84634

GTX84634 WB Image

WB analysis of HEK293T cells transfected with CYP2E1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84634 CYP2E1 antibody [5F11].
Loading : 5 ug per lane

Anti-CYP2E1 antibody [5F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84634

GTX84634 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84634 CYP2E1 antibody [5F11].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519