APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.25-0.5μg/ml |
| 0.5-1μg/ml |
| 1-3μg/1x10⁶ cells |
| 0.1-0.5μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄
Preservative
0.05mg Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 2 subfamily E member 1 , CPE1 , CYP2E , P450-J , P450C2E
Cellular Localization
Endoplasmic reticulum membrane , Mitochondrion inner membrane
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04494 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX04494 CYP2E1 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH6.0)
Dilution : 1μg/ml
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GTX04494 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX04494 CYP2E1 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH6.0)
Dilution : 1μg/ml
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GTX04494 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX04494 CYP2E1 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH6.0)
Dilution : 1μg/ml
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GTX04494 WB Image
WB analysis of various sample lysates using GTX04494 CYP2E1 antibody.
Lane 1: Rat liver tissue lysates
Lane 2 : Mouse liver tissue lysates
Dilution : 0.5 μg/mL
Loading : 50 μg
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GTX04494 FACS Image
FACS analysis of SiHa cells using GTX04494 CYP2E1 antibody.
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells
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GTX04494 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX04494 CYP2E1 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH6.0)
Dilution : 1μg/ml
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REFERENCE
There are currently no references for CYP2E1 antibody (GTX04494). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CYP2E1 antibody (GTX04494). Be the first to share your experience with this product.
