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CYP2E1 antibody

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04495
Anti-CYP2E1 antibody used in Western Blot (WB). GTX04495

Cat. No. GTX04495

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($449)
See all CYP2E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.05-0.2μg/ml
IHC-P 3-5μg/ml
ELISA 1:32000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RKVIKNVAEVK, from the internal region of the protein sequence according to NP_000764.1

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 2 subfamily E member 1 , CPE1 , CYP2E , P450-J , P450C2E

Cellular Localization

Endoplasmic reticulum membrane , Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04495

GTX04495 IHC-P Image

IHC-P analysis of human liver tissue using GTX04495 CYP2E1 antibody.
Antigen retrieval : Steamed antigen retrieval with citrate buffer (pH 6)
Dilution : 3.8 μg/ml

Anti-CYP2E1 antibody used in Western Blot (WB). GTX04495

GTX04495 WB Image

WB analysis of human liver tissue lysates using GTX04495 CYP2E1 antibody.
Dilution : 0.05 μg/mL
Loading : 35 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 449