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CYP2E1 antibody

Anti-CYP2E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32546
Anti-CYP2E1 antibody used in Western Blot (WB). GTX32546
Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546
Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

Cat. No. GTX32546

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CYP2E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 2 subfamily E member 1 , CPE1 , CYP2E , P450-J , P450C2E

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP2E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32546

GTX32546 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32546 CYP2E1 antibody.
Blue : DAPI

Anti-CYP2E1 antibody used in Western Blot (WB). GTX32546

GTX32546 WB Image

WB analysis of various sample lysates using GTX32546 CYP2E1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

GTX32546 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32546 CYP2E1 antibody.
Dilution : 1:100

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

GTX32546 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX32546 CYP2E1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Chang YY et al. J Agric Food Chem 2013; 61 (46) : 11016-24 Hepatoprotection of noni juice against chronic alcohol consumption: lipid homeostasis, antioxidation, alcohol clearance, and anti-inflammation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399