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CYP2E1 antibody

Anti-CYP2E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32546
Anti-CYP2E1 antibody used in Western Blot (WB). GTX32546
Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546
Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

Cat No. GTX32546

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:100
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome P450 family 2 subfamily E member 1 , CPE1 , CYP2E , P450-J , P450C2E

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion inner membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CYP2E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32546

GTX32546 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32546 CYP2E1 antibody.
Blue : DAPI

Anti-CYP2E1 antibody used in Western Blot (WB). GTX32546

GTX32546 WB Image

WB analysis of various sample lysates using GTX32546 CYP2E1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

GTX32546 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX32546 CYP2E1 antibody.
Dilution : 1:100

Anti-CYP2E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32546

GTX32546 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32546 CYP2E1 antibody.
Dilution : 1:100

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329