Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
*Rat liver microsome
Predict Reactivity
Human, Chimpanzee, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.03mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CYP3A4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
CP33 antibody, CP34 antibody, CYP3A antibody, CYP3A3 antibody, CYPIIIA3 antibody, CYPIIIA4 antibody, HLP antibody, MGC126680 antibody, NF-25 antibody, P450C3 antibody, P450PCN1 antibody, CYP3A4 antibody, cytochrome P450 HLp antibody, cytochrome P450 3A4 antibody, cytochrome P450 3A3 antibody, albendazole sulfoxidase antibody, albendazole monooxygenase antibody, glucocorticoid-inducible P450 antibody, "P450-III, steroid inducible antibody", taurochenodeoxycholate 6-alpha-hydroxylase antibody, cytochrome P450-PCN1 antibody, quinine 3-monooxygenase antibody, cytochrome P450 NF-25 antibody, nifedipine oxidase antibody, "cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody", "cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody", "cytochrome P450, family 3, subfamily A, polypeptide 4 antibody"
Cellular Localization
Endoplasmic reticulum membrane , Microsome membrane
Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Database
Research Area
