APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CYP3A4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10728927
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 3 subfamily A member 4 , CP33 , CP34 , CYP3A , CYP3A3 , CYPIIIA3 , CYPIIIA4 , HLP , NF-25 , P450C3 , P450PCN1
Cellular Localization
Endoplasmic reticulum membrane , Microsome membrane
Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Database
Research Area
REFERENCE
There are currently no references for CYP3A4 antibody [N2C2], Internal (GTX105871). Be the first to share your publications with this product.
REVIEW
CYP3A4 antibody [N2C2], Internal
Cat. No. GTX105871
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 23-Mar-2012
Application Tested :
WB
Sample Species :
Rat
Sample :
Liver Microsome
Amount used :
30μg
Blocking :
5% skim milk, 4°C, overnightHr
Primary Antibody :
1:1000, 4°C, 4Hr
Figure Note :
Rats do not express 3A4. Rats do express others in the CYP family which this antibody cross-reacts with. That explains the cross-reaction with other rat CYP families (only we dont know which exact one!).
![Anti-CYP3A4 antibody [N2C2], Internal used in Western Blot (WB). GTX105871](/upload/website/support/rewardsprogram/normal/genetex_GTX105871_WB_19080419_4c36bf27-1518-4493-8575-b44a130aa4bb5392.jpg "Anti-CYP3A4 antibody [N2C2], Internal used in Western Blot (WB). GTX105871")