GeneTex
United States (US)

CYP3A4 antibody [N2C2], Internal

Cat No. GTX105871

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

*Rat liver microsome

Predict Reactivity

Human, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP3A4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CP33 antibody, CP34 antibody, CYP3A antibody, CYP3A3 antibody, CYPIIIA3 antibody, CYPIIIA4 antibody, HLP antibody, MGC126680 antibody, NF-25 antibody, P450C3 antibody, P450PCN1 antibody, CYP3A4 antibody, cytochrome P450 HLp antibody, cytochrome P450 3A4 antibody, cytochrome P450 3A3 antibody, albendazole sulfoxidase antibody, albendazole monooxygenase antibody, glucocorticoid-inducible P450 antibody, "P450-III, steroid inducible antibody", taurochenodeoxycholate 6-alpha-hydroxylase antibody, cytochrome P450-PCN1 antibody, quinine 3-monooxygenase antibody, cytochrome P450 NF-25 antibody, nifedipine oxidase antibody, "cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody", "cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody", "cytochrome P450, family 3, subfamily A, polypeptide 4 antibody"

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.

Database

Research Area

REVIEW

CYP3A4 antibody [N2C2], Internal Cat No. GTX105871

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : Liver Microsome
Amount used : 30μg
Blocking : 5% skim milk, 4°C, overnightHr
Primary Antibody : 1:1000, 4°C, 4Hr
Figure Note : Rats do not express 3A4. Rats do express others in the CYP family which this antibody cross-reacts with. That explains the cross-reaction with other rat CYP families (only we dont know which exact one!).
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169