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CYP3A43 antibody

KO/KD Overexpression
Anti-CYP3A43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117120
Anti-CYP3A43 antibody used in IHC (Paraffin sections) (IHC-P). GTX117120
Anti-CYP3A43 antibody used in Western Blot (WB). GTX117120

Cat. No. GTX117120

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CYP3A43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP3A43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617497

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 3 subfamily A member 43

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CYP3A43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117120

GTX117120 ICC/IF Image

CYP3A43 antibody detects CYP3A43 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYP3A43 protein stained by CYP3A43 antibody (GTX117120) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-CYP3A43 antibody used in IHC (Paraffin sections) (IHC-P). GTX117120

GTX117120 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CYP3A43(GTX117120) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CYP3A43 antibody used in Western Blot (WB). GTX117120

GTX117120 WB Image

CYP3A43 antibody detects CYP3A43 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP3A43 antibody (GTX117120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wei QY et al. Int J Mol Sci 2022; 24 (1) Effects of CYP3A43 Expression on Cell Proliferation and Migration of Lung Adenocarcinoma and Its Clinical Significance.
Application : WB
Reactivity : Human
Michielin F et al. Cell Rep 2020; 33 (9) : 108453 The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.
Application : ICC/IF
Reactivity : Human
Giantin M et al. Sci Rep 2019; 9 (1) : 19672 Functional impact of cytochrome P450 3A (CYP3A) missense variants in cattle.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169