APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:250 |
| 1:100-1:500 |
| 2μg per 10⁶ cells |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues E S R D E T V S G A, conjugated to ovalbumin.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_11165127
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 3 subfamily A member 7 , CP37 , CYPIIIA7 , P-450(HFL33) , P-450111A7 , P450-HFLA
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
![Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463](/templates/default/js/lightbox/images/loading.gif "Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463")
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GTX79463 WB Image
WB analysis of 25 ug of HepG2 (lane 1), Hela (lane 2) and HT29 (lane 3) cell lysates using GTX79463 CYP3A7 antibody [F19 P2 H2].
Dilution : 1:1000
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GTX79463 FACS Image
FACS analysis of HepG2 cells using GTX79463 CYP3A7 antibody [F19 P2 H2] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX79463 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX79463 CYP3A7 antibody [F19 P2 H2].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX79463 FACS Image
FACS analysis of Hela cells using GTX79463 CYP3A7 antibody [F19 P2 H2] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4ºC
Fixation : 2% paraformaldehyde
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GTX79463 IHC-P Image
IHC-P analysis of human kidney tissue using GTX79463 CYP3A7 antibody [F19 P2 H2].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100
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GTX79463 FACS Image
FACS analysis of HT29 cells using GTX79463 CYP3A7 antibody [F19 P2 H2] compared to an isotype control (blue).
Dilution : 1:40 for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX79463 IHC-P Image
IHC-P analysis of human liver tissue using GTX79463 CYP3A7 antibody [F19 P2 H2].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
|
REFERENCE
There are currently no references for CYP3A7 antibody [F19 P2 H2] (GTX79463). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CYP3A7 antibody [F19 P2 H2] (GTX79463). Be the first to share your experience with this product.
![Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_1940_WB_w_23061322_356.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_212_FACS_w_23061322_979.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_1397_IHC-P_w_23061322_911.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_211_FACS_w_23061322_430.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_1395_IHC-P_w_23061322_313.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_213_FACS_w_23061322_416.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/small/GTX79463/GTX79463_1396_IHC-P_w_23061322_366.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_1940_WB_w_23061322_356.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Western Blot (WB). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_212_FACS_w_23061322_979.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_1397_IHC-P_w_23061322_911.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_211_FACS_w_23061322_430.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_1395_IHC-P_w_23061322_313.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_213_FACS_w_23061322_416.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in Flow cytometry (FACS). GTX79463")
![Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463](/upload/website/prouct_img/normal/GTX79463/GTX79463_1396_IHC-P_w_23061322_366.webp "Anti-CYP3A7 antibody [F19 P2 H2] used in IHC (Paraffin sections) (IHC-P). GTX79463")