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CYP46A1 antibody [N1C1]

Anti-CYP46A1 antibody [N1C1] used in Western Blot (WB). GTX115492
Anti-CYP46A1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115492
Anti-CYP46A1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115492

Cat. No. GTX115492

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CYP46A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP46A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173413

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 46 subfamily A member 1 , CP46 , CYP46

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CYP46A1 antibody [N1C1] used in Western Blot (WB). GTX115492

GTX115492 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (GTX115492) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYP46A1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115492

GTX115492 IHC-P Image

CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
CYP46A1 stained by CYP46A1 antibody [N1C1] (GTX115492) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CYP46A1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115492

GTX115492 IHC-P Image

CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
CYP46A1 stained by CYP46A1 antibody [N1C1] (GTX115492) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169