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CYP4F3 antibody, N-term

Anti-CYP4F3 antibody, N-term used in Western Blot (WB). GTX47110
Anti-CYP4F3 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47110

Cat No. GTX47110

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

OVCAR-3

PROPERTIES

Form

Liquid

Buffer

1x PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human CYP4F3

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome P450 family 4 subfamily F member 3 , CPF3 , CYP4F , LTB4H

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-CYP4F3 antibody, N-term used in Western Blot (WB). GTX47110

GTX47110 WB Image

WB analysis of OVCAR-3 cells using GTX47110 CYP4F3 antibody at 0.2-1μg/ml.

Anti-CYP4F3 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47110

GTX47110 IHC-P Image

IHC-P analysis of human liver tissue using GTX47110 CYP4F3 antibody at 1:100. Left to right:DAPI, CYP4F3, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409