GeneTex
United States (US)

CYP7B1 antibody [C3], C-term

GTX104776_39687_20141128_IHC_19011506_283.jpg
GTX104776_WB_19011506_110.jpg
GTX104776_40205_IFA_19011506_561.jpg

Cat No. GTX104776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYP7B1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome P450 Family 7 Subfamily B Member 1,Cbas3,Cp7B,Spg5A,Cyp7B1

Cellular Localization

Endoplasmic reticulum membrane , Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. [provided by RefSeq]

Database

DATA IMAGES
GTX104776_39687_20141128_IHC_19011506_283.jpg

GTX104776 IHC-P Image

CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human liver carcinoma.
CYP7B1 antibody [C3], C-term (GTX104776) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104776_WB_19011506_110.jpg

GTX104776 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
10% SDS PAGE
GTX104776 diluted at 1:1500

GTX104776_40205_IFA_19011506_561.jpg

GTX104776 ICC/IF Image

CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYP7B1 protein stained by CYP7B1 antibody [C3], C-term (GTX104776) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169