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CYR61 antibody [HL2144]

Recombinant antibody Orthogonal
Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122
Anti-CYR61 antibody [HL2144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638122
Anti-CYR61 antibody [HL2144] used in IHC (Paraffin sections) (IHC-P). GTX638122
Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122
Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122

Cat. No. GTX638122

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2144

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CYR61 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CYR61

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular communication network factor 1 , CYR61 , GIG1 , IGFBP10

Cellular Localization

Secreted

Background

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122

GTX638122 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CYR61 antibody [HL2144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638122

GTX638122 ICC/IF Image

CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis.
Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min.
Green: CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CYR61 antibody [HL2144] used in IHC (Paraffin sections) (IHC-P). GTX638122

GTX638122 IHC-P Image

CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122

GTX638122 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CYR61 antibody [HL2144] used in Western Blot (WB). GTX638122

GTX638122 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations