CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis.
Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min.
Green: CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.