CYR61 antibody [N1C3]

Name: CYR61 antibody [N1C3]
Brand: GeneTex
SKU: GTX103669
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CYR61 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103669

Anti-CYR61 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103669

Anti-CYR61 antibody [N1C3] used in Western Blot (WB). GTX103669

Cat. No. GTX103669

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all CYR61 products

View product citations for antibody GTX103669 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

This antibody's mouse and rat reactivity is based on IHC-P testing and may not be suitable for mouse and rat WB assay.

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYR61. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular communication network factor 1 , CYR61 , GIG1 , IGFBP10

Cellular Localization

Secreted

Background

CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.[supplied by OMIM]

Database

Gene ID: 3491 CCN1
UniProt: O00622 CCN1

Research Area

DATA IMAGES

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects secreted CYR61 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CYR61 stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects secreted CYR61 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CYR61 stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects CYR61 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103669 ICC/IF Image

CYR61 antibody [N1C3] detects CYR61 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYR61 protein stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects secreted CYR61 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CYR61 stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103669 IHC-P Image

GTX103669 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [N1C3] (GTX103669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103669 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [N1C3] (GTX103669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects secreted CYR61 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CYR61 stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Poli G et al. FASEB J 2018; : fj201801028RR Human fetal adrenal cells retain age-related stem- and endocrine-differentiation potential in culture.

Application : IHC-P

Reactivity : Human

Shih YC et al. Circ Res 2018; (Epub) Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.

Application : WB

Reactivity : Human

Chen MC et al. Int J Mol Sci 2017; 18 (12) Simvastatin Inhibits Cell Proliferation and Migration in Human Anaplastic Thyroid Cancer.

Application : WB

Reactivity : Human

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