GeneTex
United States (US)

CYR61 antibody [N1C3]

Orthogonal
GTX103669_40150_20151030_IHC-P_R_19011506_754.jpg
GTX103669_40150_20150924_IFA_19011506_842.jpg
GTX103669_40150_WB_Brefeldin A_19011720_808.jpg

Cat No. GTX103669

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa (1X Brefeldin A for 24hr)

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYR61. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCN1 antibody, GIG1 antibody, IGFBP10 antibody, CYR61 antibody, CCN family member 1 antibody, insulin-like growth factor-binding protein 10 antibody, "cysteine-rich, anigogenic inducer, 61 antibody", IGFBP-10 antibody, cysteine-rich heparin-binding protein 61 antibody, IGF-binding protein 10 antibody, IBP-10 antibody, protein CYR61 antibody, "cysteine-rich, angiogenic inducer, 61 antibody"

Cellular Localization

Secreted

Background

CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX103669_40150_20151030_IHC-P_R_19011506_754.jpg

GTX103669 IHC-P Image

CYR61 antibody [N1C3] detects CYR61 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
CYR61 antibody [N1C3] (GTX103669) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103669_40150_20150924_IFA_19011506_842.jpg

GTX103669 ICC/IF Image

CYR61 antibody [N1C3] detects CYR61 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYR61 protein stained by CYR61 antibody [N1C3] (GTX103669) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX103669_40150_WB_Brefeldin A_19011720_808.jpg

GTX103669 WB Image

Orthogonal

CYR61 antibody detects CYR61 protein by western blot analysis. Un-treated (-) and treated (+, 1x Brefeldin A treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody (GTX103669) diluted by 1:1000.

REFERENCE
REVIEW

CYR61 antibody [N1C3] Cat No. GTX103669

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : mice ventricle
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, overnightHr
Figure Note : ISO: Isoproterenol
SDS
Glycerol.pdf
Thimerosal.pdf