GeneTex
United States (US)

CaMK1 antibody [N2C3]

GTX106082_IFA_19011506_713.jpg
GTX106082_WB_19011506_871.jpg
GTX106082_39911_IHC_M_19011506_902.jpg

Cat No. GTX106082

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAMKI antibody, MGC120317 antibody, MGC120318 antibody, CAMK1 antibody, caM-KI antibody, calcium/calmodulin-dependent protein kinase type 1 antibody, caMKI-alpha antibody, caM kinase I alpha antibody, calcium/calmodulin-dependent protein kinase I antibody

Background

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106082_IFA_19011506_713.jpg

GTX106082 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CaMK1(GTX106082) antibody at 1:200 dilution.

GTX106082_WB_19011506_871.jpg

GTX106082 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX106082 diluted at 1:1000

GTX106082_39911_IHC_M_19011506_902.jpg

GTX106082 IHC-P Image

CaMK1 antibody [N2C3] detects CaMK1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CaMK1 antibody [N2C3] (GTX106082) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf