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CaMKI delta antibody [N1C3]

Anti-CaMKI delta antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108565
Anti-CaMKI delta antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108565
Anti-CaMKI delta antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108565
Anti-CaMKI delta antibody [N1C3] used in Western Blot (WB). GTX108565
Anti-CaMKI delta antibody [N1C3] used in Western Blot (WB). GTX108565

Cat. No. GTX108565

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CaMKI delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKI delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase ID , CKLiK , CaM-K1 , CaMKID

Cellular Localization

Cytoplasm (Probable) , Nucleus (Probable)

Background

This gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CaMKI delta antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108565

GTX108565 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CAMK1D(GTX108565) antibody at 1:200 dilution.

Anti-CaMKI delta antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108565

GTX108565 IHC-P Image

CaMK1D antibody [N1C3] detects CaMK1D protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
CaMK1D antibody [N1C3] (GTX108565) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKI delta antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108565

GTX108565 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using CAMK1D(GTX108565) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKI delta antibody [N1C3] used in Western Blot (WB). GTX108565

GTX108565 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKI delta antibody [N1C3] (GTX108565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKI delta antibody [N1C3] used in Western Blot (WB). GTX108565

GTX108565 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX108565 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169