GeneTex
United States (US)

CaMKI delta antibody [N1C3]

GTX108565_40016_WB_M_brain_19011507_125.jpg
GTX108565_WB_19011507_965.jpg
GTX108565_IFA_19011507_823.jpg
GTX108565_IHC_19011507_603.jpg
GTX108565_40016_20150417_IHC_M_19011507_394.jpg

Cat No. GTX108565

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKI delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium/Calmodulin Dependent Protein Kinase Id,Cklik,Cam-K1,Camkid,Camk1D

Cellular Localization

Cytoplasm (Probable) , Nucleus (Probable)

Background

This gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108565_40016_WB_M_brain_19011507_125.jpg

GTX108565 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX108565 diluted at 1:1000

GTX108565_WB_19011507_965.jpg

GTX108565 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX108565 diluted at 1:1000

GTX108565_IFA_19011507_823.jpg

GTX108565 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CAMK1D(GTX108565) antibody at 1:200 dilution.

GTX108565_IHC_19011507_603.jpg

GTX108565 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using CAMK1D(GTX108565) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108565_40016_20150417_IHC_M_19011507_394.jpg

GTX108565 IHC-P Image

CaMK1D antibody [N1C3] detects CaMK1D protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
CaMK1D antibody [N1C3] (GTX108565) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf