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United States (US)

CaMKI delta antibody

Anti-CaMKI delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105169
Anti-CaMKI delta antibody used in Western Blot (WB). GTX105169
Anti-CaMKI delta antibody used in IHC (Paraffin sections) (IHC-P). GTX105169

Cat No. GTX105169

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKI delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium/Calmodulin Dependent Protein Kinase Id,Cklik,Cam-K1,Camkid,Camk1D

Cellular Localization

Cytoplasm (Probable) , Nucleus (Probable)

Background

This gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CaMKI delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105169

GTX105169 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CAMK1D(GTX105169) antibody at 1:200 dilution.

Anti-CaMKI delta antibody used in Western Blot (WB). GTX105169

GTX105169 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX105169 diluted at 1:500

Anti-CaMKI delta antibody used in IHC (Paraffin sections) (IHC-P). GTX105169

GTX105169 IHC-P Image

CaMK1D antibody detects CAMK1D protein at nucleus on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
CaMK1D antibody (GTX105169) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf