APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.84 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CaMKI gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949805
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium/calmodulin dependent protein kinase IG , CLICK3 , CLICKIII , VWS1 , dJ272L16.1
Cellular Localization
Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Cell membrane; Peripheral membrane protein
Background
This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX107499 WB Image
CaMKI gamma antibody detects CaMKI gamma protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
CaMKI gamma antibody (GTX107499) dilution: 1:5000
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GTX107499 IHC-P Image
CaMKI gamma antibody detects CAMK1G protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
CaMKI gamma antibody (GTX107499) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX107499 WB Image
Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX107499 diluted at 1:1000
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GTX107499 WB Image
CaMKI gamma antibody detects CaMKI gamma protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
CaMKI gamma antibody (GTX107499) dilution: 1:5000
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GTX107499 WB Image
CaMKI gamma antibody detects CaMKI gamma protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10 % SDS-PAGE
CaMKI gamma antibody (GTX107499) dilution: 1:5000
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REFERENCE
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REVIEW
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