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CaMKII alpha antibody [6G9]

Anti-CaMKII alpha antibody [6G9] used in Western Blot (WB). GTX22725
Anti-CaMKII alpha antibody [6G9] used in Western Blot (WB). GTX22725

Cat. No. GTX22725

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G9

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($359)
See all CaMKII alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1 μg/mL
ICC/IF 1:100
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. It does not detect the ~60 kDa beta subunit in either phosphorylation state. Immunofluorescence staining of CaM kinase II in rat hippocampal cells with GTX22725 results in intense staining of cell somas and neuropil areas, while nuclei are only lightly stained.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Partially purified, rat CaM kinase II.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin-dependent protein kinase II alpha , PK2CDD , PKCCD

Cellular Localization

Cell junction, synapse,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-CaMKII alpha antibody [6G9] used in Western Blot (WB). GTX22725

GTX22725 WB Image

WB analysis of unphosphorylated and phosphorylated rat brain homogenate (left and right lanes respectively) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1] or GTX22725CaMKII alpha antibody [6G9] .

Anti-CaMKII alpha antibody [6G9] used in Western Blot (WB). GTX22725

GTX22725 WB Image

WB analysis of whole cell extracts (30 μg lysate) of Rat Brain (Lane 1), and Mouse Brain (Lane 2) using GTX22725 CaMKII alpha antibody [6G9].
Dilution : 0.5-1 μg/ml

REFERENCE

Application Reference

Librizzi L et al. Epilepsia 2021; 62 (7) : 1715-1728 Peripheral blood mononuclear cell activation sustains seizure activity.
Application : WB
Reactivity : Pig

Clone Reference

Nichols GS et al. Neural Plast 2015; 2015:819257 Hunting increases phosphorylation of calcium/calmodulin-dependent protein kinase type II in adult barn owls.
Lee SJ et al. Nature 2009; 458 (7236):299-304 Activation of CaMKII in single dendritic spines during long-term potentiation.
Ouyang Y et al. J Neurosci 1999; 19 (18):7823-33 Tetanic stimulation leads to increased accumulation of Ca(2+)/calmodulin-dependent protein kinase II via dendritic protein synthesis in hippocampal neurons.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359