GeneTex
United States (US)

CaMKII alpha antibody, Internal

GTX88413_966_IHC-P_18122102_893.jpg
GTX88413_965_IHC-P_18122102_565.jpg
GTX88413_57_FACS_18122102_791.jpg
GTX88413_126_ICC-IF_18122102_525.jpg
GTX88413_127_ICC-IF_18122102_553.jpg
GTX88413_2342_WB_18122102_202.jpg

Cat No. GTX88413

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.3μg/ml
ICC/IF 10μg/ml
IHC-P 3.75μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the membrane of U2OS and U251 cells.

IHC-P
Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88413-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_741960.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium/Calmodulin Dependent Protein Kinase Ii Alpha,Camka,Camkiinalpha,Mrd53,Camk2A

Cellular Localization

Postsynaptic lipid rafts.

Background

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES
GTX88413_966_IHC-P_18122102_893.jpg

GTX88413 IHC-P Image

IHC-P analysis of human cortex using GTX88413 CaMKII alpha antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88413_965_IHC-P_18122102_565.jpg

GTX88413 IHC-P Image

IHC-P analysis of human adrenal gland using GTX88413 CaMKII alpha antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88413_57_FACS_18122102_791.jpg

GTX88413 FACS Image

FACS analysis of PFA fixed Kelly cells using GTX88413 CaMKII alpha antibody, Internal.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10µg/ml

GTX88413_126_ICC-IF_18122102_525.jpg

GTX88413 ICC/IF Image

ICC/IF analysis of PFA fixed U251 cells using GTX88413 CaMKII alpha antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX88413_127_ICC-IF_18122102_553.jpg

GTX88413 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX88413 CaMKII alpha antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX88413_2342_WB_18122102_202.jpg

GTX88413 WB Image

WB analysis of human cerebellum (A), mouse (B) and rat (C) brain lysate using GTX88413 CaMKII alpha antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329