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CaMKII alpha antibody

Anti-CaMKII alpha antibody used in IHC (Frozen sections) (IHC-Fr). GTX127939
Anti-CaMKII alpha antibody used in Western Blot (WB). GTX127939
Anti-CaMKII alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127939

Cat. No. GTX127939

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CaMKII alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of rat CaMKII alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885661

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin-dependent protein kinase II alpha , PK2CDD , PKCCD

Cellular Localization

Cell junction , synapse , presynaptic cell membrane

Background

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

Database

Research Area

DATA IMAGES

Anti-CaMKII alpha antibody used in IHC (Frozen sections) (IHC-Fr). GTX127939

GTX127939 IHC-Fr Image

CaMKII alpha antibody detects CaMKII alpha protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CaMKII alpha protein stained by CaMKII alpha antibody (GTX127939) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CaMKII alpha antibody used in Western Blot (WB). GTX127939

GTX127939 WB Image

CaMKII alpha antibody detects CaMKII alpha protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
10 % SDS-PAGE
CaMKII alpha antibody (GTX127939) dilution: 1:1000

Anti-CaMKII alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127939

GTX127939 ICC/IF Image

CaMKII alpha antibody detects CaMKII alpha protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII alpha stained by CaMKII alpha antibody (GTX127939) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Yu N et al. Neurosci Bull 2023; 39 (4) : 617-630 Basal Forebrain Cholinergic Innervation Induces Depression-Like Behaviors Through Ventral Subiculum Hyperactivation.
Application : IHC-Fr
Reactivity : Mouse
Wu D et al. Mol Neurodegener 2023; 18 (1) : 23 Targeting a vulnerable septum-hippocampus cholinergic circuit in a critical time window ameliorates tau-impaired memory consolidation.
Application : IHC-Fr
Reactivity : Mouse
Dongjian Cao et al. Commun Biol 2021; 4 (1) : 1224 Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization.
Application : IHC-Fr
Reactivity : Mouse
Zou L et al. Nat Commun 2021; 12 (1) : 5871 Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology.
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169